Received 5 August 2014
Received in revised form 9 August 2014
Accepted 19 August 2014
Available online 30 August 2014
Antimicrobial activity, Cucumis anguria, Callus extract, Minimum Inhibitory Concentration.
Francis Shantha Margaret, Balasubrahmaniyan Ranjini, Arunachalam Ambethkar, Chockalingam Uma Maheswari and Natesan Selvaraj*
Associate Professor in Botany, Plant Tissue Culture Laboratory, Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli 620 023, Tamil Nadu.
*Corresponding author; email@example.com; Mob: +919442411555
ABSTRACT: The in vitro antimicrobial activity of Cucumis anguria L. callus extract was studied against selected pathogenic fungi and bacteria, by well diffusion method. Callus was extracted using Chloroform, Acetone, Methanol, Benzene, and Petroleum ether. The callus extracted with methanol was most effective than other solvents used against pathogenic bacteria Escherichia coli, Streptococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella paratyphi and fungi Fusarium oxysporum, Aspergillus fumigatus, Candida albicans and Aspergillus flavus, Aspergillus niger. Acetone extracts showed maximum inhibitory activity (34.9mm) against Streptococcus aureus , whereas for fungal methanol extract exhibiting highest zone of inhibition (8.6 mm) against Candida albicans. Chloroform extract showed moderate range of inhibitory activity against bacterial pathogens and fungal pathogens. Benzene and Petroleum ether extracts were inefficient in inhibiting the bacterial and fungal growth, when compared to other extracts.