Article History

Received 08 August 2015
Received in revised form 12 August 2015
Accepted 10 September 2015
Available online 30 September 2015

Keywords

Ricinus communis, modified MS medium, multiple shoot induction, Pluronic F 68, Silver Nitrate, Acclimatization.

A comparative study of PGRs and Pluronic F68, a surfactant on in vitro regeneration of castor (Ricinus communis L.) using shoot tip and cotyledonary node explant

Ganesh Kumari Kulathuran1* and Jayabalan Narayanasamy2

1Department of Plant Science, Bharathidasan University, Trichy, Tamil Nadu, India.
2Emeritus Professor, Department of Plant Science, Bharathidasan University, Trichy, Tamil Nadu, India.

*Corresponding author : gkumarimahesh@rediffmail.com

ABSTRACT: A highly efficient protocol for in vitro propagation of Ricinus communis L. was developed that consisted of initiation, bud proliferation, shoot elongation and rooting stages. At the initiation stage, cotyledonary nodes (CN) and Shoot tip (ST) (6 - 8 mm) were used as explants to obtain growth. Among the different concentrations of cytokinins tested, multiple shoot proliferation was obtained on modified Murashige and Skoog (mMS) medium supplemented with Thidiazuron (TDZ) 0.3 mg/l. Regular subcultures were done to prevent phenol exudation which otherwise leads to the loss of cultures. Our study also showed the response of castor cultivar TMV6 using various amino acids, carbon sources and medias for efficient multiple shoot production. After the shoot proliferation stage, Pluronic F68 was added in the range of 0.1–1.0 mg/l together with the combination of 0.3 mg/l Thidiazuron for the production of more number of multiple shoots. Up to 52.7 shoots per cotyledonary node explant and 39.4 shoots per shoot tip explant were produced. A third stage with 0.3 mg/l Gibberelic acid (GA3) together with 0.6 mg/l Pluronic F68 (PF 68) enabled shoot elongation. Roots were induced on mMS basal medium supplemented with 1.5 mg/l indole-3-butyric acid (IBA) with 0.6 mg/l Silver Nitrate (AgNO3). About 73% of rooted shoots survived after acclimatization. The procedure described herein may prove useful for clonal micropropagation of selected genotypes of castor.

 

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