Article History

Received 05 May 2017
Received in revised form 12 May 2017
Accepted 21 May 2017
Available online 30 May 2017

Keywords

Agarose gel electrophoresis; Bottle gourd; Genetic purity; SSR markers.

FINGERPRINTING OF BOTTLE GOURD (LAGENARIA SICERARIA) HYBRIDS AND THEIR PARENTAL LINES USING MICROSATELLITE MARKERS AND THEIR UTILIZATION IN GENETIC PURITY ASSESSMENT

Tejaswini Ghatage1, Shubham Dhurwe2, Rama Mishra3, Pulak Das1* and Ambresh K. Verma1

1Adithya Biotech lab and Research Pvt Ltd, Raipur - 492 099, Chhattisgarh, India.

2Kalyan PG College, Bhilai, Chhattisgarh, India.

3KIIT University, Bhuvaneshwar, Odisha, India.

*Corresponding author : pulak16@gmail.com

ABSTRACT: Use of morphological differences, between true hybrids and off types in grow out test (GOT) for genetic purity analysis, are not always apparent and cannot be recognised easily. Further, morphological traits are costly, tedious to score and environment sensitive. Alternatively, it is suggested that recent breakthrough in molecular markers can be employed in genetic purity analysis. Microsatellite markers are used for fingerprinting of bottle gourd hybrids in order to assess variation within parental lines. Fifty two microsatellite markers were employed to fingerprint to three hybrids and their parental lines. Three Simple Sequence Repeat (SSR) markers were found to be polymorphic across the hybrids and to produce unique fingerprints for each of the three hybrids. These highly informative primers not only differentiated the parent genotypes, but also confirmed the parentage of the true hybrids. The microsatellite marker, LSR 47 amplified alleles specific to different parental lines of AS lattu; likewise, LSR 77 primer for AS 3417 and LSR 15 primer for AS 606. Results indicate that microsatellite procedures are excellent genomic tools for parentage confirmation and hybridity determination, and may also enhance efficiency of breeding programmes. This technique is simple to use, more accurate and not affected by environment when compared with GOT.

 

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