Article History

Received 02 January 2018
Received in revised form 15 January 2018
Accepted 28 January 2018
Available online 30 January 2018


Brinjal, ISSR, RAPD, Polymorphism, Fingerprinting.


Shikha Shrivastava, Somya Sharma and Pulak Das*

Adithya Biotech lab and Research Pvt Ltd, Raipur, 492099, Chhattisgarh, India.

*Corresponding author :

ABSTRACT: Brinjal is a vegetable that is extensively grown in India. The maintenance of genetic purity of brinjal plant is a matter of great concern for the breeders. The recent advances in molecular markers prove its efficiency in the genetic purity analysis. Fingerprinting with molecular markers allows precise and rapid variety identification. The genuiness of a hybrid is one of the most important characteristics of good quality seed. Conventionally used grow out test (GOT) for genetic purity analysis requires use of morphological differences between true hybrids and off types which is not always apparent and can’t be recognized easily. Further, morphological traits are costly, tedious to score and very sensitive towards environment. Eight parental lines of brinjal (i.e. AB54, AB73, AB52, AB 04) were used for production of 4 hybrids. Total 18 ISSR and 8 RAPD primers were selected for the study of 8 parental lines, among them 8 ISSR and 3 RAPD primers were found to be polymorphic across the parental lines, out of which 1 ISSR and 2 RAPD primers produced unique fingerprinting across the hybrids. The ISSR marker, UBC 820 amplified alleles specific to different parental lines for hybrids (ABH1001 and ABH1002), likewise RAPD primer B18 for ABH 1003 and B5 for ABH 1004. Thus, our study showed that aid of molecular markers are more reliable, highly efficient and reproducible for assessing fingerprinting of brinjal commercial hybrid seeds with more accuracy.


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