Article History

Received 04 December 2017
Received in revised form 25 January 2018
Accepted 15 February 2018
Available online 28 February 2018


Cashew Nut Shell Oil, HPTLC, GC-MS.

Analysis of bioactive components in ethanolic extract of Cashew Nut Shell Oil using HPTLC and GC-MS techniques

Dorathy Selva Jeba Pritha S1* and Karpagam S2

1Research Scholar, Department of Botany, Queen Mary’s College, Chennai -600 004.

 2Associate Professor, Department of Botany, Queen Mary’s College, Chennai-600 004.

*Corresponding author :

ABSTRACT: The extraction of phytochemicals from the plant materials has been reported to have tremendous research interest and proved great potential. This work highlights the importance of Cashew Nut Shell Oil (CNSO) and determines the bioactive compounds. The separation of bioactive constituents done by HPTLC method on silica gel precoated aluminium plates by using solvent system :Toluene : ethyl acetate : glacial acetic acid (9:1:0.2). Scanning of HPTLC fingerprint at 254nm, 366nm and visible light. The bands showed the presence of green, blue, violet and brown revealed the presence of steroids, terpenoids and saponins after spraying with Vanillin- sulphuric acid. In GC-MS analysis different peaks with high and low molecular weight were determined and identified the presence of thirty bioactive compounds. The results of the present study explore the presence of potential phytocomponents that serve in qualitative, quantitative analysis and appropriate for standardization of the extract for therapeutic properties.


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