Article History

Received 06 July 2018
Received in revised form 18 July  2018
Accepted 27 July  2018
Available online 30 July  2018

Keywords

Anticancer, Cell line, Histochemistry, In-Vitro Propagation.

Tissue Culture, Histochemical, Phytochemical analysis and Anti-cancer activity study on a Traditional Medicinal Plant - Spharenthus indicus L.

Bagath Singh C1*, G.A.I Ebenezer1, D. Jesubalan2 and M.S.Sundari3

1Department of Botany, Madras Christian College, Tambaram, Chennai – 600 059.

2Botanical Survey of India, Western Regional Centre, Pune - 411 001, India.

3Department of Botany, S.P. Pune University, India.

*Corresponding author : rcbagath007@gmail.com

ABSTRACT: Spharenthus indicus L., a multipotential plant belonging to the family Asteraceae was undertaken for various investigations. Explants like leaf, stem and axillary bud were attempted for their morphogenetic potential by culturing on MS medium supplemented with different plant PGR hormones such as 2,4-D, 2 4 5-T, BAP, IBA and KIN . Leaf disc explants cultured on MS medium supplemented with 2 4-D induce callus in 15 days. The stem explants remains recalcitrant and it is interesting to observe that the axillary buds induced callus with Kinetin treatment followed by rhizogenesis with BAP. Phytochemical tests with leaf sample reveals the presence of alkaloids, terpenoids, flavonoids and phenols, as well as primary metabolites like protein and carbohydrates. Methanolic leaf extract with different concentrations viz., 7.8g/ml, 15.6 g/ml, 31.2 g/ml, 62.5 g/ml, 125 g/ml, 250 g/ml, 500 g/ml and 1000 g/ml were tried for toxicity effect on Lung cancer cell line A549. MTT assay adopted 1000 g/ml concentration reveals 81% anticancer activity.

 

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