Article History

Received 20 November 2014
Received in revised form 30 November 2014
Accepted 05 December 2014
Available online 10 December 2014

Keywords

Gymnema sylvestre, qualitative and quantitative analysis, in vitro propagation, nodal explants.

Phytochemical analysis and in vitro propagation of Gymnema sylvestre R.Br. a valuable medicinal plant

Chiluveru Mohan*, Aireddy Karnakar, Surapaneni Sateesh, Bhukya Rama Devi, Pamuraju Manjula, Dasari Sreekanth, Bittlingu Keerthi and Prathibha Devi

Biotechnology and Molecular Genetics Laboratory, Department of Botany, Osmania University, Hyderabad - 500007, India.

*Corresponding author: ch.mohan85@gmail.com

ABSTRACT: The present paper reports the phytochemical and micropropagation studies of a threatened plant, Gymnema sylvestre. Gymnema sylvestre which belongs to the family Asclepiadaceae is a perennial slow growing medicinal woody climber commonly called as “Gudmar”. There is a growing demand for leaves of G. sylvestre in the pharmaceutical trade due to its use as a remedy for diabetes and also as a tonic of the nerves and as a laxative. Propagation of this plant is often hard and expensive. In the present study the qualitative analysis confirmed the presence of various phytochemicals like alkaloids, flavonoids, phenols, tannins, terpenoids and glycosides. Quantitative estimation of flavonoids and phenols was also carried out. In vitro propagation is an alternative method of propagation of the threatened and endangered plant which can aid its conservation. The nodal explants were cultured on MS medium containing different concentration and combinations of growth regulators like 6-benzylaminopurine (BAP) and indoleacetic acid (IAA). Multiple shoot buds were regenerated successfully from the nodal explants which were efficiently rooted on strength MS medium supplemented with IBA. The regenerated plantlets were successfully transferred to the glasshouse, acclimatized and transferred to the field.

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